inkqubo yokusebenza ye-oligo yesiko

inkqubo yokusebenza ye-oligo yesiko,
inkqubo ye-oligo yokuxhasa izixhobo,

Icandelo le-Elution

1. Umqolo we-8 imingxuma yokutofa ulwelo, enokuthi itofwe ngaxeshanye, kwaye ulwelo oluphambili lunokulawulwa ngabanye.
2. Izikhundla zebhotile ezi-5 ze-reagent, ibhotile nganye ixhotyiswe ngebhotile ye-reagent ye-4L, oko kukuthi, inokuhambelana neentlobo ezi-5 ze-reagents.
3. Isixhobo siqhutywa yi-positive pressure liquid, efanelekileyo kwaye iguquguqukayo ukulawula izinga lokuhamba kwamanzi.
4. Isixhobo sixhotyiswe nge-96-well plate position, kwaye uhlobo lweplate luchazwe ngumthengi.
5. Ipleyiti enamanzi anzulu angama-96 inokubekwa phantsi kwepleyiti ukuqokelela ulwelo, okanye inokudityaniswa ngokuthe ngqo kumbhobho ukukhupha ulwelo olulahlwayo.
6. Kwinxalenye yesoftware, unokukhetha uhlobo lwe-reagent kunye nobungakanani be-reagent yokongeza iisampulu, ixesha linokusetwa, isixhobo soxinzelelo sinokusetwa, ixesha loxinzelelo linokulawulwa, kwaye amanyathelo anokubekwa kuwo. baleka ngokuqhubekayo.Iintlobo ezi-2 zeeresiphi.

Inxalenye yokucoca

1. Isixhobo sinokuzalisa iikholamu ze-96 c18 ngexesha elinye.
2. Isixhobo esiqhelekileyo sixhotyiswe ngamachweba amahlanu e-reagent, i-tube holder ye-centrifuge, kwaye umnini unokuthwala ubuninzi be-96 primer centrifuge tubes.
3. Isixhobo samkela inkqubo yolawulo oluzimeleyo lwe-axis ezintathu, ngokuchaneka okuphezulu kunye nokuphindaphinda okulungileyo.
4. Isixhobo samkela uhlobo lokucoca ukufunxa.
5. Izicwangciso zesoftware ziguquguqukayo kwaye kulula ukusebenza.
6. Intloko yokufunxa ixhotyiswe ngesixhobo sokuva inqanaba lolwelo esinokuziva ngokuzenzekelayo umphakamo wenqanaba lolwelo.

Umbhobho inxalenye

1. Ipleyiti ye-96-well kwi-tube ye-centrifuge.
2. I-automatic pipette tip: i-primer nganye inokwahlulwa ibe yimichilo emi-2 kakhulu.
3. X, Y, Z intshukumo ye-axis.
4. Uluhlu lwevolumu yokufunxa (5-200ul).
5. Xa uhlula kwiityhubhu ezininzi.
6. Iimfuno zexesha.
7. Inokudityaniswa nethempleyithi yokulinganisa, umsebenzi okhawulezileyo wempazamo, kukho indawo yokumisa phakathi eBomvu ebonisa ukuba ivolumu iyonke ayanelanga kwaye kufuneka igqitywe.
Kukho iingcebiso ezibomvu, ezityheli kunye neziluhlaza zokubala umthamo: umthubi uthetha ukuba umthamo uncinci, unokuguqula ngesandla umthamo, akukho mfuneko yokubala, uhlaza luthetha ukuba luqhelekileyo.
8. Ulwazi olufana nenombolo yekholomu, inani leetyhubhu, kunye nomthamo wetyhubhu nganye iboniswa xa ugcwalisa isampuli nganye.Ubume bubonisa ukuba iiprimers zahluliwe kwaye azihlulwanga, ukulungiswa kokumisa okuzenzekelayo phambi kokunikezela ngakunye.

Inkqubo yesiko ye-oligo yokusebenzela ye-oligo inkqubo ye-aupport izixhobo, ine-fuction ye-Pepetting, ukuxuba, ukungcangcazela, ukufudumeza kunye nokunye.