ISikhululo soMbhobho sokuSebenza esiZizenzekelayo esigcweleyo esinokudluliselwa kolwelo
Uphawu
1. Indawo yokusebenzela inokubeka iliso kuyo yonke inkqubo yokufunxa kunye nenaliti ngexesha langempela ngokuseta iiparamitha ukuze kufunyanwe izinto ezingaqhelekanga ezifana nokufunxa okuncinci, ukuvuza kunye nokuvalwa kwehlwili kwinkqubo yokufunxa kunye nokukhupha, kwaye uzilungise ngeenkqubo zonyango ezihambelanayo.
2. Indawo yokusebenza ithatha isixhobo sokufunxa esisuka kumazwe angaphandle, esinokuqonda iimpawu zokuchaneka okuphezulu kunye nengcebiso enye eneentloko ezininzi.
3. Ubungakanani obubambeneyo, obuguquguqukayo kumsebenzi kunye nobuhle obuyilwe ukuba bulingane kwiihoods zomsi eziqhelekileyo kunye neekhabhathi zokhuseleko lwendalo.Imisebenzi yokufaka imibhobho emininzi kwiyunithi enye.
4. Ulawulo lwe-PLC, lulula, lunembile kwaye kulula ukulusebenza, olunonxibelelwano lomzobo lomsebenzisi.
5. Umbhobho ozenzekelayo
Kwiimeko ezingajongwanga, ingcebiso ingatshintshwa ngokuzenzekelayo ukugqiba umsebenzi wokulinga, ukukhulula umfunisi kunye nokuqinisekisa ukuzinza kunye nokuveliswa kobuchule bokulinga.
6. Iqonga lokubhobhoza eliguquguqukayo
Amacwecwe asebenzayo anokubekwa ngokweemeko zokuvavanya umthengi ukufezekisa i-pipetting ngokukhawuleza phakathi kwe-microplates.
7. Ukuchaneka okuphezulu kwepayipi
Ukuchaneka kombhobho kuyisalathisi esibalulekileyo sokusebenza kwendawo yokusebenzela yombhobho, ukusetyenziswa kweengcebiso zeDicken kunye nokutywinwa kakuhle kuqinisekisa ukuchaneka, ukuthembeka kunye nokuveliswa kwakhona.
Inkcazo
1. Ngeengcebiso ze-TECAN zepayipi, ukuchaneka kwe-ultra-high pipetting, iintlobo ezimbini zeengcebiso: enye i-200ul kunye ne-1000ul enye.Isofthiwe ichaza ngokuzenzekelayo umthamo we-pipetting liquid kwaye isebenzisa i-tip ye-1000ul xa umthamo we-pipetting liquid idlula i-200ul, kwaye isebenzisa i-tip ye-200ul xa umthamo we-pipetting liquid ungaphantsi kwe-200ul.
2. Jonga kwi-TECAN iingcebiso zombhobho ukuchaneka njengoko kungezantsi.
Qaphela: Ezi parameters kukuchaneka kuvavanywa ngeengcebiso ze-TECAN pipette.
| DiTi (µl) | Umthamo (µl) | Ukukhupha | Ukuchaneka kwendawo (A) | Ukuchaneka (CV) |
| 10 | 1 | One* | ≦5% | ≦6% |
| 10 | 5 | One* | ≦2.5 % | ≦1.5% |
| 10 | 10 | One* | ≦1.5% | ≦1% |
| 50 | 5 | One* | ≦5% | ≦2% |
| 50 | 10 | One* | ≦3% | ≦1% |
| 50 | 50 | One* | ≦2% | ≦0.75% |
| 200 | 10 | One* | ≦5% | ≦2% |
| 200 | 50 | One* | ≦2% | ≦0.75% |
| 200 | 200 | One* | ≦1% | ≦0.75% |
| 1000 | 10 | One* | ≦7.5% | ≦3.5% |
| 1000 | 100 | One* | ≦2% | ≦0.75% |
| 1000 | 1000 | One* | ≦1% | ≦0.75% |
| 1000 | 100 | Ezininzi** | ≦3% | ≦2% |
3. Ukusebenza kwesoftware
Umsebenzisi ubeka umnini ngemithamo eyahlukeneyo yeetyhubhu nakweyiphi na indawo kwaye emva koko aqinisekise ubudlelwane bendawo kwisoftware kwaye umsebenzi unokuqalisa.
4. Ngomsebenzi wokuva inqanaba lolwelo, inokuqonda inqanaba lolwelo kwiindidi ezahlukeneyo zetyhubhu ukukhusela ngokufanelekileyo ukuphuphuma kwamanzi.
