Umgaqo we-Oligo Synthesizer
Kwimimandla yebhayoloji yemolekyuli kunye nophando lwemfuzo, ukukwazi ukwenza iDNA kudlala indima ebalulekileyo.I-DNA synthesis ibandakanya imveliso eyenziweyo yeemolekyuli zeDNA ngokucwangcisa iinucleotides ngendlela ethile.Ukufezekisa oku, izazinzulu zithembele kwisixhobo esinamandla esaziwa njenge-oligonucleotide synthesizer, eyaziwa ngokuba yi-DNA synthesizer.
I-oligonucleotide synthesizer sisixhobo esiphucukileyo esenza ngokuzenzekelayo iimolekyuli ezimfutshane ze-DNA ezibizwa ngokuba yi-oligonucleotides.Le misonto imfutshane ye-DNA iqhele ukuba yi-10 ukuya kwi-100 ye-nucleotide ubude kwaye iyimfuneko yokwakha kwiindidi ezahlukeneyo zezicelo ezibandakanya i-polymerase chain reaction (PCR), i-gene synthesis, i-genetic engineering, kunye ne-DNA sequencing.
Ii-oligonucleotide synthesizers zisebenza kumgaqo wobuchule owaziwa ngokubai-slid-phase synthesis.Le ndlela yaqalwa ngoovulindlela nguGqr. Marvin Caruthers owawongwa ngembasa yeNobel ngeminyaka yoo-1970 kwaye iye yaphuculwa ekuhambeni kweminyaka ukuze kuphuculwe ulandelelwano lweDNA.I-Oligonucleotide synthesis iqhutyelwa ngokongezwa kwinqanaba le-nucleotide intsalela kwi-5'-terminus yekhonkco elikhulayo de ulandelelwano olufunwayo luhlanganiswe.Udibaniso ngalunye lubizwa ngokuba ngumjikelo we-synthesis kwaye lubandakanya ukusabela kweekhemikhali ezine:
Inyathelo 1: Ukuvala (detritylation)----------Inyathelo lesi-2: Ukudibanisa----------Inyathelo 3: Ukucofa------------Inyathelo lesi-4: I-oxidation
Le nkqubo iphinda iphindwe kwi-nucleotide nganye de kube ulandelelwano olufunwayo lufunyenwe.Kwi-oligonucleotides ende, lo mjikelo unokufuna ukuphindaphinda amaxesha amaninzi ukuze udibanise ulandelelwano lonke. Ukukwazi ukulawula ngokuchanekileyo inyathelo ngalinye lomjikelo we-synthesis kubalulekile kwi-oligonucleotide synthesizer.Ii-reagents ezisetyenzisiweyo, ezifana ne-nucleotides kunye ne-activators, kufuneka zibe kumgangatho ophezulu wokuqinisekisa ukudibanisa ngokuchanekileyo kunye nokusebenza kakuhle.Ukongeza, ii-synthesizers zifuna ulawulo oluchanekileyo oluphezulu lobushushu kunye nezinye iimeko zokusingqongileyo ukukhuthaza ukusabela okunqwenelekayo kokudibanisa kunye nokuthintela ukuphendula okungafunekiyo.
Nje ukuba i-oligonucleotide yenziwe ngokupheleleyo, ngokuqhelekileyo icatshulwe kwinkxaso eqinileyo kwaye ihlanjululwe ukususa nawaphi na amaqela asele akhuselayo okanye ukungcola.I-oligonucleotides ehlanjululweyo sele ilungele izicelo ezisezantsi.
Ukuqhubela phambili kwezobuchwepheshe kwenze ukuba kuphuhliswe i-oligonucleotide synthesizer ephezulu ekwazi ukudibanisa amakhulu okanye amawaka e-oligonucleotides ngaxeshanye.Ezi zixhobo zisebenzisa iteknoloji ye-microarray-based synthesis, eyenza abaphandi bavelise ngokukhawuleza iilayibrari ezinkulu ze-oligonucleotide kwiinjongo ezahlukeneyo zophando.
Isishwankathelo, imigaqo esemva kwe-oligonucleotide synthesized synthesizers ijikeleza kubuchule bokwenziwa kwesigaba esiluqilima, esibandakanya ukongezwa ngokwenyathelo lenucleotides kwinkxaso eqinileyo.Ulawulo oluchanekileyo lomjikelo we-synthesis kunye nee-reagents ezikumgangatho ophezulu ziyimfuneko kwi-synthesis echanekileyo kunye nokusebenza kakuhle.I-Oligo synthesizer idlala indima ebalulekileyo kuphando lwe-DNA, ivumela izazinzulu ukuba zivelise i-oligonucleotides yesiko kwiinkqubo ezahlukeneyo, igalelo kwinkqubela phambili kwi-biotechnology kunye nophando lwemfuza.
Ixesha lokuposa: Aug-01-2023