Zombini i-DNA kunye ne-RNA synthesis isekwe kwisicwangciso esiliqilima se-synthesis kunye ne-phosphoramidite chemistry, i-synthesizer ye-DNA ingasetyenziselwa ukudibanisa i-RNA okanye i-RNA analogue ngaphandle kohlengahlengiso olungakumbi, kunye ne-reagents kwi-DNA synthesis inokusetyenziswa ngokuthe ngqo kwi-RNA kunye ne-nucleic acids eyenziweyo. 'ukuhlanganiswa.Njengoko kuboniswe kwi-Figure 1, i-2'-hydroxy kwi-RNA phosphoramidite ikhuselwe kunye neqela elikhuselayo le-silyl, oko kukuthi i-t-butyldimethylsilyl (TBDMS), ukuthintela ukuphendula kwecala kwiqela le-2'-hydroxy ebuthathaka.Iqela elikhulu le-TBDMS lithintele ukusabela phakathi kwe-phosphoramidite kunye neqela le-5'-hydroxy kwinkxaso eqinile, kwaye kufuneka ixesha elide lokudibanisa ukuze kuqondwe ukwanelisa ukudibanisa okusebenzayo.
Ngokufana ne-DNA synthesis, i-RNA ehlanganisiweyo yaqala yacandwa kwinkxaso eqinileyo nge-aminolysis, emva koko iqela le-TBDMS lacandwa yi-tetrabutylammonium fluoride (TBAF) okanye i-trimethylamine trihydrofluoride.I-RNA ekrwada inokucocwa ngokuphinda kuhlaziywe etywaleni nakwi-HPLC.
Umzobo 1. Ubume beekhemikhali zeebhloko zokwakha ezisisiseko kwi-DNA kunye ne-RNA synthesis.
a) dABz phosphoramidite kunye b) rABz 2'-OTBDMS phosphoramidite.
Ukuphuhliswa kwechiza le-siRNA kufuna ukwakheka kwe-analogues ye-RNA yemveli ukwandisa ukuhambelana kwe-biocompatibility, i-2'-hydroxy kwi-RNA inokutshintshwa yi-MeO, i-F kunye neqela le-MOE, kunye ne-nucleic acid etshixiweyo (LNA) inika ukusebenza kakuhle kunyango lwe-RNA. (Umfanekiso 2).Ezi phosphoramidites zinika imisebenzi efana ne-DNA yohlobo lwe-phosphoramidites kwi-synthesis, kunye nenkqubo yokusebenza kunye nokuhlanjululwa kwezi nucleic acids ezingekho zendalo zifana ne-DNA yokuzalwa.
Umzobo 2. Ubume beekhemikhali zeebhloko zokwakha kwiziyobisi ze-siRNA.a) dABz 2-MeO phosphoramidite;b) dABz 2-F phosphoramidite;c) dABz 2-MOE phosphoramidite kunye d) dABz Itshixiwe iphosphoramidite.
Iiphosphoramiditeszibalulekile kwi-synthesis yezakhi zofuzo, kubandakanywa ikakhulu i-DNA kunye neentsapho ze-RNA kunye ne-derivatives yazo, yonke i-phosphoramidite ngasentla sinokunikezela kwiphakheji.
Sihlala siphucula ukusebenza kweemveliso zethu, sifezekisa inkqubo yethu yokuvelisa kunye nokufumana iinkcukacha ngokufanelekileyo.Asikuboneleli kuphela ngeemveliso ezikumgangatho ophezulu, kodwa sikwabonelela ngoqeqesho kunye nenkonzo.Sixhotyiswe ngabasebenzi bolawulo lobuchwephesha kunye neqela lobuchwephesha eligqwesileyo kunye nabasebenzi bolondolozo ukubonelela abathengi bethu ngenkonzo egqibeleleyo emva kokuthengisa.
Ixesha lokuposa: Aug-09-2022