Imigibe yeemolekyuli ye-phosphoramidite kunye ne-reagents

Isicelo:

I-Molecular Trap isetyenziselwa ukubhengeza umkhondo wamanzi kwi-reagents kunye ne-amidite, ekuqaleni yayiyilwe ukuhlanganiswa kwe-oligonucleotides.Ifanelekile, ayinathuli, kwaye ayinaflaneli.Inokongezwa kwiintlobo ezahlukeneyo ze-solvents kunye nezisombululo ze-organic ukususa umkhondo wamanzi.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ingcaciso yeMveliso

Kwi-ABI kunye ne-MilliGen / PerSeptive DNA yovavanyo lwe-synthesis, ipakethe ye-sub-sieve inokugcina umthamo wamanzi kwi-nitrile kunye neebhotile ze-activator ngaphantsi kwe-10 ppm, ngaphandle kokukhathazeka malunga ne-valve okanye i-valve ye-throttle ihlulwe kwi-sub-sieve.Uthuli okanye i-lint kwi-packaging bag ivaliwe.Ingasetyenziselwa ngokuthe ngqo ukuchithwa kwamanzi kwi-500 ml, i-1L, i-2 L kunye nezinye iibhotile ze-solvent, kunye neenkcukacha zepakethi engaphantsi kwe-sieve nazo zingenziwa ngokwezifiso.

Oku kulandelayo kubonisa umphumo oguqukayo wokuchithwa kwamanzi we-10 g i-molecular trap kwi-500ml, i-1L, i-4L iisampulu ze-nitrile ezinomxholo ohlukeneyo wamanzi.

Thelekisa umgibe wemolekyuli wokungenisa elizweni

500 ml 197 ppm ACN
Ixesha(h)	0	24	48	72	96
HonyaBio	197	33	16.5	6.5	6
Amanye Amazwe	197	43	27	15	15

1 L 143 ppm ACN
Ixesha(h)	0	24	48	72	96
HonyaBio	143	48	32	20	15
Amanye Amazwe	142	47	36	23	15

4 L 141 ppm ACN
Ixesha(h)	0	24	48	72	96
HonyaBio	141	95	94	84	73
Amanye Amazwe	141	96	95	85	72

Imiyalelo kunye nokusetyenziswa

I-molecular trap i-vacuum-packed, kwaye kufuneka ivulwe nokuba isetyenzisiwe, kwaye ukutywinwa kwebhotile ye-reagent kufuneka kuqinisekiswe ngexesha lokusetyenziswa.
I-2g yesihluzo se-24 h inokunciphisa i-165 ppm isiqulatho samanzi kwi-500 ml ye-nitrile ukuya kwi-105 ppm.
I-5 g yesefu ye-24 h inokunciphisa i-172 ppm isiqulatho samanzi kwi-500 ml ye-nitrile ukuya kwi-58 ppm.
I-10 g isisefo 24 h Inokunciphisa umthamo wamanzi we-166 ppm kwi-1 L nitrile ukuya kwi-68 ppm.
I-20 g sub-sieve inokunciphisa i-162 ppm umthamo wamanzi kwi-4 L nitrile ukuya kwi-109 ppm nge-24 h.

Sincoma i-2 g ye-subsieve kwiibhotile ze-reagent ye-50-250ml, i-5g kwiibhotile ze-reagent ye-250-500ml, i-10g ye-500-1000ml iibhotile ze-reagent, kunye ne-20g ye-1000-2000ml iibhotile ze-reagent.

Isiqinisekiso

Iphakheji nganye yesikrini esisezantsi iphantsi kolawulo olungqongqo lwekhwalithi ngexesha lenkqubo yokuvelisa.Kwaye kuyimfuneko ukuba abathengi bajonge ukupakishwa ngaphambi kokusetyenziswa:

Okokuqala, qinisekisa ukuba i-vacuum yokupakisha icocekile.Nakuphi na ukuvuza kwe-vacuum okanye ukungena komoya kuya kunciphisa ukusebenza kwemveliso.

Okwesibini, lumka xa uvula ukupakishwa ukukhusela ifilimu yokupakisha engaphantsi kwesikrini ukuba ingakrwelwa, kwaye ujonge ukuba ifilimu ikhona Kukho ukuvuza.

Unyango lwasemva kokusetyenziswa

Ayiyotyhefu okanye ayinabungozi, iyakungcoliswa yimichiza kunye nezinyibilikisi ezidibana nazo kwaye kufuneka zilahlwe njengenkunkuma engcolileyo emva kokusetyenziswa.

Imodeli kunye nokusetyenziswa

Ngaphambili siye sanikezela ngeengxowa ze-sub-sieve ngobukhulu be-2 g, 5 g, 10 g, kunye ne-20 g., kunye nezinye iinkcukacha zingenziwa ngokwezifiso ngokweemfuno zabathengi.

Umgibe we-molecular ulungele ukuvezwa kwexesha elide kwi-nitrile, kwaye unokusetyenziselwa i-acetic acid, i-ether, i-acetic ether, i-butyl acetate, utywala, i-isopropanol, i-methanol, i-butanol, i-Phenol, i-pyridine, i-hydrochloric acid, i-nitric acid, i-phosphoric acid. , i-asidi ye-sulfuric, i-methyl chloride, i-nitrogen methyl imidazole, njl.

Akunakufaneleka ukubonakaliswa kwexesha elide kwi-tetrahydrofuran, toluene, methyl formamide (DMF), i-methyl methyl amide (DMAc), i-N-methylpyrrolidone (NMP) kunye nezinye izisombululo.